01. Studying skin’s cellular tolerability
when cosmetics are administered
02. Analysis of an active agent’s penetration
03. Evidence of an anti-aging mechanism
04. Evaluation of a soothing product designed for over-reactive skin
05. Evaluation of solar protection
06. Evaluation of a depigmenting or pro pigmenting effect
07. Evaluation of anti-inflammatory effect
08. Objectification of elastin synthesis
09. Objectification of glycosaminoglycans synthesis
10. Evaluation of cellular desquamation
11. Study of cellular re-growth on an experimental model following superficial burns:
12. Anti-radical effect on an experimental model highlighting oxidative stress:
13. Evaluation of an anti-pollution effect (environmental or tobacco):
14. Evaluation of an anti-cellulite response:
I. Evaluation of cosmetic and dermatologic products carried out on human skin kept in a life-like state
Skin capillaries (hemalun - eosine)
Histological visualisation of skin capillaries (hemalun - eosine)
- Experimental model for over-reactive skin will be realized either:
- - by epidermal irritation due to the application of lauryl sulphate
- - establishment of semi-quantitative scores concerning the dermis and epidermis
- - dose of cytokines
- - by the application of a neuromediator (P substance)
- - establishment of semi-quantitative scores for the evaluation of the oedema and dermal vasodilation.
- - Cytokines assay
Experimental model of radiation of the skin by UV A and B
Evaluation of solar protection
- Evaluation of UV B protection:
- Histology (quantification of sun-burnt cells and cellular alterations on the epidermis)
- Cellular viability (MTT test)
- Dosage of hydroperoxides in order to show evidence of anti-radical activity
- Dosage of nitrites
- Dosage of cytokines
- Evaluation of UV A protection:
- - Histology of elastic fibers and of collagen (quantification analysis made from computer images)
- Dosage of cytokines.
Skin phototype III-IV maintained in survival for an evaluation of changes in depigmentation or conversely skin phototype II for the evaluation of propigmentation
Evaluation of a depigmenting effect
Histologcial evaluation of the number of melanic pigments in the melanocytes and the adjacent keratinocytes (coloration by Fontana-Masson)
- Evaluation of the melanin content after coloration with Fontana-Masson
- Configuration of pigmentation scores
- Evaluation of melanogenesis (DOPA reaction) by quantification of melanocyte count
Experimental model of inflammation after the application of neuro-mediators or of irritants
Evaluation of anti-inflammatory effect
Histologcial evaluation of the number of melanic pigments in the melanocytes and the adjacent keratinocytes (coloration by Fontana-Masson)
- Evaluation of an anti-inflammatory reaction :
- Histology
- Immunohistochemistry with the aid of antibodies anti-lymphocytes T, B
- Cytokin assay
Objectification of elastin synthesis
- Remainder of skin cultures, kept in survival conditions
- Soluble elastin assay (Fastin Elastin Assay)
Objectification of glycosaminoglycans synthesis
- Extraction of glycosaminoglycans from the dermis by a papain
- Dose depending on Blyscan Glucosaminoglycan Assay.
Update : december 2010
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