- Evaluation de la cohésion du stratum cornéum :

• Evaluation of stratum corneum cohesiveness 
• Establishment of semi-quantitative scores
• Comparative study with the desquamation obtained by a standardized product of reference (glycolic acid 10%)
• Histological analysis (hemalun-eosine)
• Immunohistochemical analysis of a marker of differentiation with the aide of an anti-involucrine antibody

- Evaluation of the final differentiation of the stratum corneum
Immunohistochemical analysis of a marker of differentiation with the aide of an anti-involucrine antibody

Immunohistochemical analysis

Immunohistochemical analysis of a marker of differentiation with the aide of an anti-involucrine antibody

Analysis of exfoliation of the skin

Desquamated skin

- Experimental model of epithelial alteration by burns after the application of agarose gel heated to 85°C

- Study of cellular rehabilitation :

Normal skin

Skin altered by burns

• Quantification of the number of altered cells by histological analysis, of cytoplasmic clarification, picnotic nucleus
  Vasodilatation analysis:
  - Morphometric measure of the capillairies` diameter (µm2)
  - Measure of percentage of dilated vessels
• Dosage of pro-inflammatory cytokines (IL1a, IL 8)

- Experimental model of cellular alteration due to oxidative stress : UVA-UVB radiation or application of xanthine oxydase/hypoxantine or hydroperoxides to generate free radicals

Anti-radical effect

Experimental model of cellular alteration due to oxidative stress 

• dosage of nitrites (NO2 en µM)
• dosage of residual carbonyls to the epidermis (test ELISA) (results recorded in DO or in µM of carbonyl)
• Evaluation of collagen cross-linking (dosage of hydroxyproline in µg/mg of proteins after denaturingby a collagenase)
• Analysis of cellular viability (viability of mitochondrial deshydrogenases) by an exclusion test MTT(mitochondrial tetrazolium test)
• Immunohistochemistry analysis of the natural destructive enzymes of oxygenized free-radicals (semi-quantitative scores) :catalase and superoxide dismutase with manganese

- Experimental model of pollution through superficial administration of hypochlorous soda, carbon particles, lead acetate

- Model of excessive exposure to tobacco amidst a smoking location (dose equivalent to 2 packs /day)

Model of excessive exposure to tobacco

Evaluation of an anti-pollution effect (environmental or tobacco).

• Dosage of carbonyl residue on epidermis (test ELISA) (results recorded in DO or in µM de carbonyl)
  
• Evaluation of collagen cross-linking (dosage of hydroxyproline after denaturing by a collagenase)

- Model serves of whole human skin, complete with hypoderm

Evaluation of an anti-cellulite response

• Dosage of AMPc, reflection of the activation of the triglyceride lipase (test EIA, noted in pmoles /ml according to the quantity of proteins)
• Dosage of glycerol freed by the adipose tissues (nmoles /g of lipids)

Update : december 2010