I-7. Experimental model of radiation of the skin by UV A and B

Evaluation of UV B protection:

• Histology (quantification of sun-burnt cells and cellular alterations on the epidermis)
• Cellular viability (MTT test)
• Dosage of hydroperoxides in order to show evidence of anti-radical activity
• Dosage of nitrites
• Dosage of cytokines

Evaluation of UV A protection:

• Histology of elastic fibers and of collagen (quantification analysis made from computer images)
• Dosage of cytokines.

Our experimental models

INDEX : experimental models

I. Cell cultures

II. Human skin kept alive

III. In vitro reconstitution of skin equivalents

IV. Scalp sample maintained in survival conditions

V. Clinical Evaluations

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