Anti-radical effect on an experimental model highlighting oxidative stress

dosage of nitrites (NO2 en µM)
dosage of residual carbonyls to the epidermis (test ELISA) (results recorded in DO or in µM of carbonyl)
Evaluation of collagen cross-linking (dosage of hydroxyproline in µg/mg of proteins after denaturingby a collagenase)
Analysis of cellular viability (viability of mitochondrial deshydrogenases) by an exclusion test MTT(mitochondrial tetrazolium test)
Immunohistochemistry analysis of the natural destructive enzymes of oxygenized free-radicals (semi-quantitative scores) :catalase and superoxide dismutase with manganese

INDEX : experimental models: I. Cell cultures; II. Human skin kept alive; III. In vitro reconstitution of skin equivalents; IV. Scalp sample maintained in survival conditions; V. Clinical Evaluations

I-14. Anti-radical effect