Evaluation of anti-elastasic activity on frozen material
Evaluation of anti-elastasic activity in tubes
Combination of anti-elastasic active with an elastase (Elastase Pig Pancrea, Synthetic Human Elastase leucocyte, N-Succinyl-Tri-Alanine-para Nitroanilide; or elastase of fibroblastic origin) in presence of a substrate
Spectrophotometric lecture of elastasic activity at 410 nm.
Evaluation of anti-elastasic activity on frozen material
Installation of frozen histological layers starting with a fragment of human skin (plastic surgery); elastase and product aimed at becoming an anti-elastasic in contact with dermal elastic fibers
Coloration of elastic fibers with (+) catechin (photographs of histological cuts);
Morphometric quantification of elastic fibers after analysing digital images (% of fibers in each cutaneous dermis, length and number of fibers).
