. Histological and
immunohistochemical analysis
.Immunohistochemical analysis of a neo-formed epithelium
. Epidermisation of equivalents by the implantation of a epidermis fragment
. Study of the differentiation of keratinocytes on reconstituted skin in vitro:
. Display of results
III. In vitro reconstitution of skin equivalent : METHOD
Exclusivité GREDECO
- of epithelial proliferation
- of epithelial differentiation
Exemple : evaluation of a product or ray meant to have healing properties
- Implementation of a dermal equivalent by mixing type 1 collagen with fibroblasts : formation of a lattice by collagen condensation in a sterilizer/drying oven, cupboard/incubator at 37°C.
- Epidermisation of equivalents by the implantation of a epidermis fragment
Epidermisation
Epidermisation of equivalents by the implantation of a epidermis fragment
- Change-out of culture medium two times per week for two weeks, while utilising different factors (EGF, hydrocortisone) to create favourable conditions for epidermal growth
- Study of the differentiation of keratinocytes on reconstituted skin in vitro:
- Inclusion on paraffin wax for standard histological studies
- Freezing to show cytokeratins by immunochemistry;
- Fixation for ultrastructural study with electron microscope - Display of results :
- Photographs using optical and electron microscopes
- Establishment of semi-quantitative scores for cytokeratin immunochemistry
Display of results
Establishment of semi-quantitative scores for cytokeratin immunochemistry
Back to index "In vitro reconstitution of skin equivalent : METHOD"
Top of page
