GREDECO, Dermatology and Cosmetology. Evaluation of cosmetic and dermatologic products carried out on human skin kept in a life-like state.

EXPERIMENTAL MODELS > Human skin maintained in survival condition [ DERMATOLOGY COSMETOLOGY ]

I. The evaluation of cosmetic and dermatologic products is carried out on human skin kept in a life-like state

Tolerance test

Studying skin’s cellular tolerability when cosmetics are administered
- Histological analysis
- Cellular viability (measuring the activity of mitochondrial deshydrogenases
- Cytokines assay (IL1, IL8, TNFa)
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Analysis of an active agent’s penetration

- HPLC assay
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03/a

Evidence of an anti-aging mechanism

- Evaluation of keratinocyte differentiation, of the stratum corneum
- Epithelial Proliferation
- Evaluation of the quality of the dermo-epidermic jonction
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03/b

Evidence of an anti-aging mechanism

- Collagen synthesis
- Evaluation of anti-elastasic activity (following application of human leucocytal elastase or after UV A radiation)
- Evaluation of anti-collagenase activity (following the application of collagenase or after UV A radiation)
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03/c

Evidence of an anti-aging mechanism

- Evaluation of metalloproteinasic activity after IL1 or UV radiation stimulates the metalloproteinase
- Evaluation of hydrating effect
- Evaluation of anti-glycation activity after experimental aging techniques obtained by UV A radiation or by the application of nicotine
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Evaluation of a soothing product designed for over-reactive skin

- Experimental model for over-reactive skin will be realized either
. by epidermal irritation due to the application of lauryl sulphate
. by the application of a neuromediator (P substance)
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Evaluation of solar protection

- Experimental model of radiation of the skin by UV A and B
. Evaluation of UV B protection:
. Evaluation of UV A protection:
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Evaluation of a depigmenting or pro pigmenting effect

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Evaluation of anti-inflammatory effect

- Experimental model of inflammation after the application of neuro-mediators or of irritants
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Objectification of elastin synthesis

- Remainder of skin cultures, kept in survival conditions
- Soluble elastin assay (Fastin Elastin Assay)
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Objectification of glycosaminoglycans synthesis

- Extraction of glycosaminoglycans from the dermis by a papain
- Dose depending on Blyscan Glucosaminoglycan Assay.
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Evaluation of cellular desquamation

- Evaluation of stratum corneum cohesiveness :
- Evaluation of the final differentiation of the stratum corneum
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Study of cellular re-growth on an experimental model following superficial burns:

- Experimental model of epithelial alteration by burns after the application of agarose gel heated to 85 ºC
- Etude de la réparation cutanée
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Anti-radical effect on an experimental model highlighting oxidative stress:

- Experimental model of cellular alteration due to oxidative stress : UVA-UVB radiation or application of xanthine oxydase/hypoxantine or hydroperoxides to generate free radicals
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